6533b825fe1ef96bd12826e9

RESEARCH PRODUCT

Influence of temperature on the calibration curves in IRMA for neuron specific enolase and its physicochemical interpretation

J.l. Moreno FrigolsaT. Peris ConejeroR. Olivares Pallerols

subject

Microbiology (medical)Immunoradiometric assayLangmuirCalibration curvebusiness.industryImmunologyEnolaseTheoretical modelsInterpretation (model theory)Immunology and AllergyMedicineBiological systembusinessStatistical softwareGamma counter

description

Abstract Background immunoradiometric assay (IRMA) is one of the principal methods used for the analytical determination of neuron specific enolase (NSE) concentration. We studied the influence of temperature on the calibration curves obtained by this method, and a physicochemical justification based on two theoretical models is proposed. Material and methods we used a commercially available RIA kit for NSE and a gamma counter. Data was analysed using Statistical software. Results and discussion activity bound to the antibody increases with temperature, producing results that are consistent with two modifications to the four parameter and Langmuir equations. Conclusions the two models used successfully reproduce the results, with the adsorption model being preferable due to its greater simplicity and clearer physical significance.

yearjournalcountryeditionlanguage
2009-03-01Revista Española de Medicina Nuclear (English Edition)
https://doi.org/10.1016/s1578-200x(09)70015-4