6533b825fe1ef96bd128286c
RESEARCH PRODUCT
Cloning and characterization of CSP37, a novel gene encoding a putative membrane protein of Candida albicans.
M V ElorzaMaria SentandreuW A FonziRafael SentandreuA IborraAlmudena NietoJ Pontonsubject
Vesicle-associated membrane protein 8HeterozygoteRecombinant Fusion ProteinsMutantGenes FungalMolecular Sequence DataBiologyMicrobiologyRetinoblastoma-like protein 1Fungal ProteinsMiceHSPA2SNAP23Candida albicansEscherichia coliAnimalsAmino Acid SequenceCloning MolecularDNA FungalMolecular BiologyGeneHSPA9Mice Inbred BALB CBase SequenceHomozygoteMembrane ProteinsSequence Analysis DNABlotting NorthernMolecular biologyPhenotypeAKT1S1Gene DeletionResearch Articledescription
In the course of an analysis of the functions and assembly of the cell wall of Candida albicans, we have cloned and characterized a gene, which we designated CSP37 (cell surface protein), encoding a 37-kDa polypeptide which is a membrane-associated protein. The gene was isolated by immunological screening of a DNA library constructed from mycelial cells with a polyclonal serum raised against cell walls of this morphology. Analysis of the nucleotide sequence of a corresponding genomic DNA fragment revealed a single open reading frame which encodes a predicted protein of 321 amino acids with no significant homology to others in the databases. Disruption of the CSP37 gene by the method described by Fonzi and Irwin (Genetics 134:717-728, 1993) eliminated expression of the Csp37 protein. The mutant strains showed no apparent defect in cell viability, growth, or cell wall assembly but displayed attenuated virulence in systemic infections induced in mice and reduced the ability to adhere to polystyrene.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-08-01 | Journal of bacteriology |