6533b825fe1ef96bd1282919

RESEARCH PRODUCT

Injectable in situ forming hydrogels based on natural and synthetic polymers for potential application in cartilage repair

Alessandro GulinoGaetano GiammonaGaetano GiammonaStefano AgnelloCalogero FioricaGiovanna PitarresiGiovanna PitarresiFabio Salvatore Palumbo

subject

chemistry.chemical_classificationIn situScanning electron microscopeGeneral Chemical Engineeringtechnology industry and agriculturehydrogels natural polymers cartilage repairmacromolecular substancesGeneral ChemistryPolymerchemistry.chemical_compoundChemical engineeringchemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoHyaluronic acidSelf-healing hydrogelsCopolymermedicineSwellingmedicine.symptomElastic modulus

description

In this work we prepared two new hyaluronic acid (HA) based in situ forming hydrogels for the potential treatment of articular cartilage damages. In particular the amino derivative of HA (HA-EDA) and its graft copolymer with α-elastin (HA-EDA-g-α-elastin) were crosslinked, in mild physiological conditions via Michael-type addition, with α,β-poly(N-2-hydroxyethyl)-dl-aspartamide (PHEA) derivatized with divinylsulfone (DV). The swelling and degradation profile of the obtained hydrogels as well as the metabolic activity of incorporated bovine articular chondrocytes were investigated. Histological analysis and scanning electron microscopy (SEM) were performed to analyze the morphology of cells after scheduled times of incubation. Moreover the influence of the α-elastin on the elastic modulus of cell free and cell containing hydrogels has been studied through atomic force microscopy (AFM). This journal is

yearjournalcountryeditionlanguage
2015-01-01RSC Advances
https://doi.org/10.1039/c4ra16411c