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RESEARCH PRODUCT
Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells
Hilda De VriesRoberta FrapolliElena GattiCamilla RecordatiMichela FrenquelliMaurizio D'incalciMarco BianchiMassimo VenturiniAntonello E. SpinelliFrancesco De MarchisLuca CrippaRenzo BoldoriniEltjona RrapajRosanna MezzapelleEugenio ScanzianiClaudio DoglioniMassimo P. CrippaChiara CeriottiLaura PeraniSilvia ValtortaLorenza PecciariniAlessandro PretiRosa Maria MorescoRosa Maria MorescoAnton Bernssubject
Mesothelioma0301 basic medicinePathologymedicine.medical_specialtyLung NeoplasmsAntineoplastic AgentsPemetrexedHMGB1DeoxycytidineArticleProinflammatory cytokineBALB/c03 medical and health sciences0302 clinical medicineImmune systemMalignant MesotheliomCell Line TumormedicineMesothelioma HMGB1 in vivo imagingcancerAnimalsHumansMesotheliomaHMGB1 ProteinCisplatinMice Inbred BALB CMultidisciplinarybiologybusiness.industryMesothelioma Malignantbiology.organism_classificationmedicine.diseaseSurvival AnalysisGemcitabine030104 developmental biologyPemetrexedCell culturemesothelioma030220 oncology & carcinogenesisbiology.proteinFemaleCisplatinBALB/cbusinessImmunocompetenceNeoplasm Transplantationmedicine.drugdescription
Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment. Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-03-10 |