6533b825fe1ef96bd12831b4
RESEARCH PRODUCT
Secretion of the rotavirus VP8* protein inLactococcus lactis
Maria Teresa GilIsabel Pérez-arellanoJavier BuesaGaspar Pérez-martínezsubject
Signal peptidebiologymedicine.diagnostic_testvirusesLactococcusProtein subunitLactococcus lactisbiology.organism_classificationmedicine.disease_causeMicrobiologyMicrobiologyCapsidWestern blotRotavirusGeneticsmedicineSecretionMolecular Biologydescription
Secretion of the VP8* subunit of the VP4 capsid protein of rotavirus by Lactococcus lactis has been achieved. For this purpose, a secretion vector has been constructed with the lactococcal signal sequence AL9 and the VP8*-encoding gene fragment. The amount of VP8* secreted by L. lactis in the culture supernatant was quantified and visualised by Western blot. Furthermore, it was shown to retain its hemagglutination capability, indicating that the conformation of the secreted peptide may be retaining its biological activity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-09-01 | FEMS Microbiology Letters |