6533b825fe1ef96bd128322b

RESEARCH PRODUCT

Multimycotoxin analysis in water and fish plasma by liquid chromatography-tandem mass spectrometry

Jordi MañesGuillermina FontJosefa TolosaEmilia Ferrer

subject

Ochratoxin AFusariumAflatoxinEnvironmental EngineeringLiquid Phase MicroextractionHealth Toxicology and Mutagenesis01 natural scienceschemistry.chemical_compound0404 agricultural biotechnologyFusariumTandem Mass SpectrometryLiquid chromatography–mass spectrometryAnimalsEnvironmental ChemistryMycotoxinChromatography High Pressure LiquidChromatographybiology010401 analytical chemistryExtraction (chemistry)Fishestechnology industry and agriculturePublic Health Environmental and Occupational Healthfood and beveragesEnvironmental Exposure04 agricultural and veterinary sciencesGeneral MedicineGeneral ChemistryEnvironmental exposureMycotoxinsbiology.organism_classification040401 food sciencePollutionBeauvericin0104 chemical scienceschemistryWater Pollutants ChemicalEnvironmental Monitoring

description

High performance liquid chromatography-mass spectrometry was used for the determination of 15 mycotoxins in water and fish plasma samples, including aflatoxins, fumonisins, ochratoxin A, sterigmatocistin, fusarenon-X and emerging Fusarium mycotoxins. In this work, dispersive liquid-liquid microextraction (DLLME) was assessed as a sample treatment for the simultaneous extraction of mycotoxins. Results showed differences in recovery assays when different extraction solvents were employed. Ethyl acetate showed better recoveries for the major part of mycotoxins analyzed, except for aflatoxins B2, G1 and G2, which showed better recoveries when employing chloroform as extractant solvent. Fumonisins and beauvericin exhibited low recoveries in both water and plasma. This method was validated according to guidelines established by European Commission and has shown to be suitable to be applied in dietary and/or toxicokinetic studies in fish where is necessary to check mycotoxin contents in rearing water and fish plasma.

yearjournalcountryeditionlanguage
2015-09-07Chemosphere
https://doi.org/10.1016/j.chemosphere.2015.11.085