6533b825fe1ef96bd1283265
RESEARCH PRODUCT
Detection of Norovirus Antigens from Recombinant Virus-Like Particles and Stool Samples by a Commercial Norovirus Enzyme-Linked Immunosorbent Assay Kit
Shoko Okitsu-negishiShigenori KamijoTakeshi TomaruWerner E.g. MüllerYuko ShimizuFumihiro YagyuTakashi SatoMichio OkameTung Gia PhanHiroshi Ushijimasubject
Microbiology (medical)GenotypevirusesEnzyme-Linked Immunosorbent AssayBiologyRecombinant virusmedicine.disease_causeSensitivity and Specificitylaw.inventionFecesfluids and secretionsVirus-like particleAntigenlawVirologyGenotypemedicineHumansChildAntigens ViralFecesCaliciviridae Infectionschemistry.chemical_classificationReverse Transcriptase Polymerase Chain ReactionNorovirusvirus diseasesInfantVirologyGastroenteritisEnzymechemistryChild PreschoolRecombinant DNANorovirusReagent Kits Diagnosticdescription
ABSTRACT The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-10-01 |