A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions

6533b825fe1ef96bd12832cc

RESEARCH PRODUCT

A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions

Vivienne Gianinazzi-pearson Eliane Dumas-gaudot Stéphanie Weidmann Benoît Valot Gwénaëlle Bestel-corre Silvio Gianinazzi Sébastien Lenogue Nardjis Amiour

subject

0106 biological sciences Proteome Computational biology Fungus Proteomics Plant Roots 01 natural sciences Biochemistry Fungal Proteins Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Mycorrhizae Botany Protein purification Medicago [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Electrophoresis Gel Two-Dimensional [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Symbiosis Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Gene Expression Profiling fungi biology.organism_classification GENOMIQUE Medicago truncatula Arbuscular mycorrhiza Proteome Functional genomics 010606 plant biology & botany

description

We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.

year	journal	country	edition	language
2004-01-01	PROTEOMICS

https://doi.org/10.1002/pmic.200300627

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