Search results for "GENOMIQUE"
showing 10 items of 10 documents
Genomics of arbuscular mycorrhizal fungi
2004
International audience
Immunity and other defenses in pea aphids, Acyrthosiphon pisum
2010
Background Recent genomic analyses of arthropod defense mechanisms suggest conservation of key elements underlying responses to pathogens, parasites and stresses. At the center of pathogen-induced immune responses are signaling pathways triggered by the recognition of fungal, bacterial and viral signatures. These pathways result in the production of response molecules, such as antimicrobial peptides and lysozymes, which degrade or destroy invaders. Using the recently sequenced genome of the pea aphid (Acyrthosiphon pisum), we conducted the first extensive annotation of the immune and stress gene repertoire of a hemipterous insect, which is phylogenetically distantly related to previously ch…
Functional genomics of arbuscular mycorrhiza : decoding the symbiotic cell programme
2004
More extensive insight into plant genes involved in the symbiotic programme of arbuscular mycorrhiza is presently being achieved by global approaches that aim to discover novel genes or subsets of genes that are essential to cell programmes in the different steps of plantfungal interactions. The strategy of functional genomics based on large-scale differential RNA expression analyses (differential-display reverse transcriptase - PCR), electronic Northerns, suppressive subtractive hybridization, DNA chips) is presented, with a focus on arbuscular mycorrhiza in Pisum sativum and Medicago truncatula. The most recent knowledge about gene networks that are modulated in roots during arbuscular …
A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions
2004
We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.
Génomique du phytoplasme de la Flavescence Dorée
2008
National audience
Diversité microbienne des sols : stratégie méthodologique, distribution spatiale et traduction en fonctionnement biologique
2011
International audience; Since the development of industrialization, urbanization and agriculture, soils have been subjected to numerous variations in environmental conditions, which have resulted in modifications of the taxonomic diversity and functioning of the indigenous microbial communities. As a consequence, the functional significance of these losses/modifications of biodiversity, in terms of the capacity of ecosystems to maintain the functions and services on which humanity depends, is now of pivotal importance. In this context, one of the main challenges in soil microbial ecology is to better understand and predict the processes that drive soil microbial diversity and the link betwe…
Genomics based approach to identify the genes involved in ipu mineralization in sphingomonas sp.sh
2010
Phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (IPU), was found to be rapidly mineralised in aFrench agricultural soil previously exposed to IPU. A bacterial strain able to metabolise IPU was isolated from this soil adapted toIPU mineralization.
Are soils suppressive to fungal diseases the sources of biocontrol agents ?
2014
Soils suppressive to soil-borne diseases are defined by a low disease incidence in spite of the presence of a virulent pathogen and a susceptible plant. In many cases, the inhibition of the disease development relies on the activity of the resident soil microbiome. Suppressiveness can be transmitted to conducive soil by mixing a small amount of suppressive soil into the conducive one. To identify microbial taxons linked to the suppressive phenotype of soils, culture independent-based methods have been employed to analyze and compare microbial diversities in two different soils suppressive (respectively conducive) to either Fusarium wilt of flax or Rhizoctonia diseases of sugar beet. Metagen…
Traduction de la métagenomique des agrosystemes en services écologiques
2011
International audience; En affectant les communautés microbiennes présentes dans le sol, les pratiques agricoles mettent en péril la biodiversité des agrosystèmes. La génomique environnementale est un nouveau moyen d’améliorer nos connaissances sur ces derniers et ce faisant, de concevoir des systèmes agricoles plus respectueux de l’environnement.
Emerging mutualisms: fungal endophytes and their relationships with mycorrhizal fungi
2011
National audience; De nombreuses maladies émergentes apparaissent qui sont dues à des champignons, aussi bien chez les plantes, les animaux que chez l’homme. Il est donc important de comprendre les mécanismes à l’origine de ces maladies, et la biologie évolutive fournit un cadre théorique indispensable, en particulier pour comprendre l’adaptation des pathogènes aux nouveaux environnements ou aux nouveaux hôtes.