6533b85dfe1ef96bd12be80c

RESEARCH PRODUCT

Functional genomics of arbuscular mycorrhiza : decoding the symbiotic cell programme

Laurent BrechenmacherVivienne Gianinazzi-pearson

subject

0106 biological sciencesGene regulatory networkPlant Science01 natural sciences[SDV.BV.BOT] Life Sciences [q-bio]/Vegetal Biology/Botanics03 medical and health sciencesGene expressionBotanyGeneComputingMilieux_MISCELLANEOUS030304 developmental biology2. Zero hungerLUERNE0303 health sciencesbiologyfungifood and beverages[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanicsbiology.organism_classificationMedicago truncatulaGENOMIQUEArbuscular mycorrhizaSuppression subtractive hybridizationDNA microarrayFunctional genomics010606 plant biology & botany

description

More extensive insight into plant genes involved in the symbiotic programme of arbuscular mycorrhiza is presently being achieved by global approaches that aim to discover novel genes or subsets of genes that are essential to cell programmes in the different steps of plant–fungal interactions. The strategy of functional genomics based on large-scale differential RNA expression analyses (differential-display reverse transcriptase - PCR), electronic Northerns, suppressive subtractive hybridization, DNA chips) is presented, with a focus on arbuscular mycorrhiza in Pisum sativum and Medicago truncatula. The most recent knowledge about gene networks that are modulated in roots during arbuscular establishment and functioning is discussed.Key words: arbuscular mycorrhiza, symbiotic programme, gene expression, pea, annual alfalfa.

https://hal.inrae.fr/hal-02672828