0000000000181461
AUTHOR
Laurent Brechenmacher
Construction and validation of cDNA-based Mt6k-RIT macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula
To construct macro- and microarray tools suitable for expression profiling in root endosymbioses of the model legume Medicago truncatula, we PCR-amplified a total of 6048 cDNA probes representing genes expressed in uninfected roots, mycorrhizal roots and young root nodules [Nucleic Acids Res. 30 (2002) 5579]. Including additional probes for either tissue-specific or constitutively expressed control genes, 5651 successfully amplified gene-specific probes were used to grid macro- and to spot microarrays designated Mt6k-RIT (M. truncatula 6k root interaction transcriptome). Subsequent to a technical validation of microarray printing, we performed two pilot expression profiling experiments usin…
Medicago truncatula gene responses specific to arbuscular mycorrhiza interactions
International audience
The role of the plant genome in modulating arbuscular mycorrhizal formation.
International audience
High-troughput TILLING to identify symbiosis-related plant gene function in arbuscular mycorrhiza
International audience
Plant genes involved in arbuscular mycorrhiza formation and functioning
Knowledge about that part of the plant genome involved in the establishment and functioning of the arbuscular mycorrhizal (AM) symbiosis is important for the basic understanding of this symbiosis. It is also essential for a ‘genes to the field’ approach based on the identification and exploitation of genes that could be central to developing sustainable plant production systems in the future.
Identification of genes activated during arbuscular mycorrhiza interactions by suppressive substractive hybridisation and cloning of differential expresed cDNA
International audience
EST sequencing for fungal genes expressed in the arbuscular symbiosis
International audience
Functional genomics of arbuscular mycorrhiza : decoding the symbiotic cell programme
More extensive insight into plant genes involved in the symbiotic programme of arbuscular mycorrhiza is presently being achieved by global approaches that aim to discover novel genes or subsets of genes that are essential to cell programmes in the different steps of plantfungal interactions. The strategy of functional genomics based on large-scale differential RNA expression analyses (differential-display reverse transcriptase - PCR), electronic Northerns, suppressive subtractive hybridization, DNA chips) is presented, with a focus on arbuscular mycorrhiza in Pisum sativum and Medicago truncatula. The most recent knowledge about gene networks that are modulated in roots during arbuscular …