6533b825fe1ef96bd12832f3
RESEARCH PRODUCT
New spectrophotometric procedure for determining cefotaxime based on derivatization with 1,2-naphthoquinone-4-sulphonate into solid-phase extraction cartridges--application to pharmaceutical and urine samples.
Adela Sevillano-cabezaPilar Campíns-falcóFrancisco Bosch-reigLuisa Gallo Martinezsubject
AdultCefotaximeChromatographyNQSGeneral ChemistryUrineCefotaximeHigh-performance liquid chromatographySensitivity and SpecificityCephalosporinschemistry.chemical_compoundchemistryPharmaceutical PreparationsSpectrophotometryStandard additionmedicineHumansIndicators and ReagentsSolid phase extractionDerivatizationChromatography High Pressure LiquidAntibacterial agentmedicine.drugNaphthoquinonesdescription
Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C18) and detected using a UV-visible detection system. Optimum conditions for this new procedure were: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time at 25 degrees C and an NQS concentration of 7.1x10(-3) mol l(-1). The accuracy and the precision of the liquid-solid procedure were tested. The procedure was used to measure cefotaxime in pharmaceutical and urine samples. The results obtained were contrasted with those reported for a HPLC method for urine samples. The generalized H-point standard additions method was used to measure cefotaxime in urine samples.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-10-01 | Journal of chromatography. B, Biomedical sciences and applications |