6533b826fe1ef96bd12834ad
RESEARCH PRODUCT
Differential miRNA expression defines migration and reduced apoptosis in follicular thyroid carcinomas.
Tomasz StokowyThomas J. MusholtSteffen HauptmannMarkus EszlingerBartosz WojtasDariusz LangeBarbara JarzabCarolina FerrazHenning DralleRalf Paschkesubject
medicine.medical_specialtyApoptosisBiologymedicine.disease_causeBiochemistryThyroid carcinomaEndocrinologyCell MovementInternal medicineCell Line TumorFollicular phasemicroRNAAdenocarcinoma FollicularmedicineHumansThyroid NeoplasmsFollicular thyroid cancerMolecular BiologyCell ProliferationCell growthCell cyclemedicine.diseaseGene Expression Regulation NeoplasticMicroRNAsEndocrinologyApoptosisCancer researchCarcinogenesisdescription
The objective of the study was to identify microRNAs (miRs) characteristic for follicular thyroid carcinoma (FTC) and to define their role in tumorigenesis. A miR-microarray study was conducted to identify miRs differentially expressed between FTCs and their surrounding tissues. Selection was further reinforced by a literature review. Four miRs were selected and confirmed by RT-qPCR: miR-146b, -183, -221 were up-regulated, whereas miR-199b down-regulated in FTCs. The influence of these miRs on cell proliferation, cell cycle, apoptosis and migration was studied in HTori and FTC-133 cells. Functional characterization suggests an impact of miR-183 and miR-146b in FTC development. Overexpression of both miRs significantly induces migration. Moreover, overexpression of miR-183 significantly represses apoptosis. MiR-199b and -221 do not have significant effects on proliferation, cell cycle, apoptosis or migration in HTori and FTC-133 cells. Our data suggest that miR-146b and miR-183 may influence FTC development through the induction of migration and apoptosis inhibition.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-08-28 | Molecular and cellular endocrinology |