6533b826fe1ef96bd1283a79

RESEARCH PRODUCT

A Comparative Study of Different Methods of Yeast Strain Characterization

Amparo QuerolDaniel RamónEladio Barrio

subject

Gel electrophoresisGeneticsMitochondrial DNAbiologyDNA–DNA hybridizationSaccharomyces cerevisiaebiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyYeastRestriction fragmentYeast in winemakingRestriction mapbiology.proteinEcology Evolution Behavior and Systematics

description

Summary An extensive survey of different methods of yeast strain identification (classical microbiological tests, whole-cell protein electrophoresis, chromosomal patterns, DNA hybridization and mitochondrial DNA restriction analysis) has been carried out in order to differentiate, with industrial purposes, strains present in the Alicante wine ecosystem. Only chromosomal patterns and mitochondrial DNA (mtDNA) restriction analysis show differences between strains. Both techniques are very complex to be used in bio technological industries. For this reason, we have developed a new, simple, unexpensive and rapid method based on mtDNA restriction analysis.

yearjournalcountryeditionlanguage
1992-08-01Systematic and Applied Microbiology
https://doi.org/10.1016/s0723-2020(11)80219-5