Development of the H-point standard additions method for coupled liquid chromatography and UV-visible spectrophotometry

6533b826fe1ef96bd1283bee

RESEARCH PRODUCT

Development of the H-point standard additions method for coupled liquid chromatography and UV-visible spectrophotometry

Pilar Campíns-falcó Rosa Herráez-hernández Francisco Bosch-reig Adela Sevillano-cabeza

subject

Chromatography medicine.diagnostic_test Elution Chemistry Analytical chemistry Biochemistry Analytical Chemistry Spectrophotometry Standard addition medicine Environmental Chemistry Deconvolution Spectroscopy Second derivative

description

Abstract This work establishes the fundamentals of the H-point standard additions method for liquid chromatography for the simultaneous analysis of binary mixtures with overlapped chromatographic peaks. The method was compared with the deconvolution method of peak suppression and the second derivative of elution profiles. Different mixtures of diuretics were satisfactorily resolved.

year	journal	country	edition	language
1992-02-01	Analytica Chimica Acta

https://doi.org/10.1016/0003-2670(92)80154-y

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