6533b826fe1ef96bd1283c15
RESEARCH PRODUCT
Coexpression of vasoactive intestinal peptide, calcitonin gene-related peptide and substance P immunoreactivity in parasympathetic neurons of the rhesus monkey lung
Eberhard WeiheD. NohrLee E. Eidensubject
medicine.medical_specialtyCalcitonin Gene-Related PeptideVasoactive intestinal peptideNeuropeptideSubstance PSubstance PCalcitonin gene-related peptideBiologyCholine O-Acetyltransferasechemistry.chemical_compoundParasympathetic Nervous SystemInternal medicinemedicineAnimalsLungNeuronsGeneral NeuroscienceImmunohistochemistryMacaca mulattaCholine acetyltransferaseEndocrinologynervous systemchemistryFluorescent Antibody Technique DirectCalcitoninCholinergicAcetylcholineVasoactive Intestinal Peptidemedicine.drugdescription
Abstract By the use of light microscopic immunohistochemistry, the present study investigates whether substance P (SP) and calcitonin generelated peptide (CGRP), which are well documented neurotransmitter candidates in primary sensory fibers, are also expressed in parasympathetic neurons of the rhesus monkey lung. A combination of double fluorescence immunohistochemistry and staining of adjacent sections revealed triple coexistence of SP, CGRP and the cholinergic co-transmitter vasoactive intestinal peptide (VIP) in a large number of neuronal cell bodies in intrinsic peribronchial ganglia. In addition, there was co-localization of SP and CGRP in choline acetyltransferase (ChAT)-positive neurons. These data suggest that SP and CGRP, in addition to their sensory role, are cholinergic cotransmitter candidates in the postganglionic parasympathetic innervation of primate lung. Co-release and co-function of SP and CGRP with VIP and acetylcholine may be important in the regulation of pulmonary physiology and in pulmonary pathophysiology.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-10-13 |