6533b826fe1ef96bd1283c2f
RESEARCH PRODUCT
A high-quality genome assembly from short and long reads for the non-biting midge Chironomus riparius (Diptera)
Thomas HankelnMarkus PfenningerSören Lukas HellmannHanno SchmidtBarbara FeldmeyerAnn-marie Waldvogelsubject
0106 biological sciencesmedicine.medical_specialtyMitochondrial DNAEcology (disciplines)ved/biology.organism_classification_rank.speciesPopulationSequence assemblyHybrid genome assemblyQH426-470Biology010603 evolutionary biology01 natural sciencesGenomeChironomidae03 medical and health sciencesMolecular geneticschironomus ripariusGeneticsmedicineAnimalseducationMolecular BiologyGenetics (clinical)030304 developmental biologyrecombination rateChironomus riparius0303 health scienceseducation.field_of_studyGenomehybrid genome assemblyved/biologyGenome ReportEvolutionary biologydescription
AbstractBackgroundChironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species.FindingsMaking use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius’ genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species and the respective recombination rate as one of the first insect recombination rates at all.ConclusionsThe genome and associated resources will be highly valuable to the broad community working with dipterans in general and chironomids in detail. The estimated recombination rate will help evolutionary biologist gain a better understanding of commonalities and differences of genomic patterns in insects.
year | journal | country | edition | language |
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2020-04-01 |