6533b826fe1ef96bd1283dbf
RESEARCH PRODUCT
Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate
S. Pea-llopisJ.b. PeaE. SanchoC. Fernández-vegaM.d. FerrandoSamuel Peña-llopisJ.b. Peñasubject
medicine.medical_specialtyEnvironmental EngineeringHealth Toxicology and MutagenesisGlutathione reductaseDrug ResistanceMedizinReductasemedicine.disease_causechemistry.chemical_compoundAnguillidaeThiocarbamatesInternal medicinemedicineEnvironmental ChemistryAnimalsMuscle Skeletalchemistry.chemical_classificationbiologyHerbicidesPublic Health Environmental and Occupational HealthGeneral MedicineGeneral ChemistryGlutathioneAzepinesgamma-Glutamyltransferasebiology.organism_classificationAnguillaPollutionGlutathioneEndocrinologyEnzymeGlutathione ReductasechemistryLiverToxicityCarbamatesHomeostasisOxidative stressdescription
Eels of species Anguilla anguilla were exposed to 5/4 LC50 (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and gamma-glutamyl transpeptidase (gamma-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-10-30 |