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RESEARCH PRODUCT
Link between Intestinal CD36 Ligand Binding and Satiety Induced by a High Protein Diet in Mice
Michèle VigierMichèle VigierDelphine OurselDanielle NavilleDanielle NavillePhilippe BesnardA. DuchamptA. DuchamptHélène PoirierGilles MithieuxGilles MithieuxRené LessireIsabelle NiotMartine BegeotMartine Begeotsubject
CD36 AntigensMaleTime FactorsAnatomy and Physiologymedicine.medical_treatmentCD36[SDV]Life Sciences [q-bio]lcsh:MedicineOleic AcidsLigandsSatiety ResponseBiochemistryJejunumFood-intakeEatingMiceOleoylethanolamidechemistry.chemical_compound0302 clinical medicineIntestinal Mucosalcsh:ScienceReceptorSalineAnimal Management2. Zero hunger0303 health sciencesMultidisciplinaryAgricultureLipidsIntestinesmedicine.anatomical_structureSatiety Response030220 oncology & carcinogenesisChain Fatty-AcidsMedicineProtein BindingResearch ArticleReceptormedicine.medical_specialtySuccinimidesTransportBiologyBody-weightAbsorption03 medical and health sciencesInternal medicinemedicineAnimalsCholesterol UptakeBiologyNutrition030304 developmental biologyEvolutionary Biologylcsh:ROleoylethanolamideGluconeogenesisProteinsSmall intestineDietMice Inbred C57BLEndocrinologyGene Expression RegulationGluconeogenesischemistryImmunologybiology.proteinRatVeterinary Sciencelcsh:QZoology[SDV.AEN]Life Sciences [q-bio]/Food and Nutritiondescription
International audience; CD36 is a ubiquitous membrane glycoprotein that binds long-chain fatty acids. The presence of a functional CD36 is required for the induction of satiety by a lipid load and its role as a lipid receptor driving cellular signal has recently been demonstrated. Our project aimed to further explore the role of intestinal CD36 in the regulation of food intake. Duodenal infusions of vehicle or sulfo-N-succinimidyl-oleate (SSO) was performed prior to acute infusions of saline or Intralipid (IL) in mice. Infusion of minute quantities of IL induced a decrease in food intake (FI) compared to saline. Infusion of SSO had the same effect but no additive inhibitory effect was observed in presence of IL. No IL- or SSO-mediated satiety occurred in CD36-null mice. To determine whether the CD36-mediated hypophagic effect of lipids was maintained in animals fed a satietogen diet, mice were subjected to a High-Protein diet (HPD). Concomitantly with the satiety effect, a rise in intestinal CD36 gene expression was observed. No satiety effect occurred in CD36-null mice. HPD-fed WT mice showed a diminished FI compared to control mice, after saline duodenal infusion. But there was no further decrease after lipid infusion. The lipid-induced decrease in FI observed on control mice was accompanied by a rise in jejunal oleylethanolamide (OEA). Its level was higher in HPD-fed mice than in controls after saline infusion and was not changed by lipids. Overall, we demonstrate that lipid binding to intestinal CD36 is sufficient to produce a satiety effect. Moreover, it could participate in the satiety effect induced by HPD. Intestine can modulate FI by several mechanisms including an increase in OEA production and CD36 gene expression. Furthermore, intestine of mice adapted to HPD have a diminished capacity to modulate their food intake in response to dietary lipids.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-25 |