Regeneration of Sideritis angustifolia (Labiatae) Plants from Single Cell Cultures

6533b826fe1ef96bd12845e4

RESEARCH PRODUCT

Regeneration of Sideritis angustifolia (Labiatae) Plants from Single Cell Cultures

Miguel Carlos Sánchez-gras Juan Segura

subject

Tissue culture Murashige and Skoog medium biology Physiology Inoculation Cell culture fungi Botany Shoot Sideritis Plant Science biology.organism_classification Agronomy and Crop Science

description

Summary Mechanically isolated cells from hypocotyl-derived calli of Sideritis angustifolia Lag. were inoculated on agar-solidified Murashige and Skoog (MS) nutrient medium containing CH and different concentrations and combinations of NAA and BA. Isolated cells underwent continued divisions giving rise to calli after 30 days of culture. Best results were obtained when medium was supplemented with 0.5 μM NAA and 4.4 or 8.8 μM BA or with 5 μM NAA and 4.4μM BA. Calli obtained in the presence of 0.5 μM NAA and 8.8 μM BA regenerated shoot-buds when transferred to MS medium without CH and supplemented or not with these growth regulators. Once shoots were excised, the calli maintained their morphogenic competence along several subcultures in hormone-free MS medium. Regenerated shoots were easily rooted on MS medium without growth regulators.

year	journal	country	edition	language
1988-08-01	Journal of Plant Physiology

https://doi.org/10.1016/s0176-1617(88)80096-8

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