0000000000598026
AUTHOR
Miguel Carlos Sánchez-gras
Regeneration of Sideritis angustifolia (Labiatae) Plants from Single Cell Cultures
Summary Mechanically isolated cells from hypocotyl-derived calli of Sideritis angustifolia Lag. were inoculated on agar-solidified Murashige and Skoog (MS) nutrient medium containing CH and different concentrations and combinations of NAA and BA. Isolated cells underwent continued divisions giving rise to calli after 30 days of culture. Best results were obtained when medium was supplemented with 0.5 μM NAA and 4.4 or 8.8 μM BA or with 5 μM NAA and 4.4μM BA. Calli obtained in the presence of 0.5 μM NAA and 8.8 μM BA regenerated shoot-buds when transferred to MS medium without CH and supplemented or not with these growth regulators. Once shoots were excised, the calli maintained their morpho…
Accumulation of monoterpenes in shoot-proliferation cultures of Lavandula latifolia Med.
Abstract We studied the effect of osmotic stress (induced by the addition of mannitol to the culture medium) and abscisic acid (ABA) addition on monoterpene accumulative capability in shoot proliferating cultures of Lavandula latifolia Med. The highest shoot elongation was achieved when the elongation medium contained sucrose (3%). Increasing the medium osmolarity or adding 25–50 μM ABA in shoot elongation cultures resulted in a significant increase in monoterpene accumulation in the regenerated shoots. These monoterpenes are qualitatively similar to those produced by the parent plants. Regulation of the conditions that limit the growth in shoot proliferation cultures of L. latifolia should…
In Vitro Propagation of Sideritis angustifolia Lag.
Summary Hypocotyl, root and cotyledon explants from 30 day old seedlings of Sideritis angustifolia Lag. were cultured on Murashige and Skoog nutrient medium. Organogenesis of roots and shoots was induced with treatments containing BA or KIN in combination with NAA, IAA or 2,4-D, however, those combinations including NAA and BA were optimal. Most regenerated shoots developed roots when transferred to medium without growth regulators. Rooted plantlets were successfully transplanted into soil.
Spermidine and morphogenesis in single cell cultures of Sideritis angustifolia lag.
Abstract Single cells from hypocotyl-derived callus of Sideritis angustifolia were evaluated for morphogenesis when cultured in either solidifier or liquid Murashige and Skoog medium supplemented with various concentrations of spermidine, naphthaeneacetic acid (NAA), benzyladenine (BA) and dicyclohexylamine (DCHA). Spermidine (Spd) did not replace the hormonal requirements for cell division and callus formation, but affected growth induced by NAA or BA. This polyamine increased the plating efficiency of cells cultured in the presence of NAA (0.05, 0.54 and 5.40 μM) or BA (0.40, 4.40 and 8.80 μM). In contrast, Spd reduced growth induced by 10.80 μM NAA, optimal auxin concentration for callus…
Micropropagation of Lavandula latifolia through nodal bud culture of mature plants
Cultures of Lavandula latifolia Medicus were established from axillary buds of mature field-grown plants. Explants were initially cultured on media with two different macronutrient combinations and benzyladenine or kinetin added either individually or with naphthaleneacetic acid. Subsequently, explants were subcultured in Murashige and Skoog medium supplemented with 20% coconut milk, 0.57 μM indoleacetic acid and 8.88 μM benzyladenine. Shoot proliferation from axillary buds was not affected by seasonal fluctuations in the stock plants but depended on the macronutrient composition and on the type and concentration of cytokinin tested. Best results were obtained in explants initially cultured…