6533b828fe1ef96bd1288202
RESEARCH PRODUCT
Accumulation of monoterpenes in shoot-proliferation cultures of Lavandula latifolia Med.
Miguel Carlos Sánchez-grasM. C. Calvosubject
Osmotic shockOsmotic concentrationMonoterpenefungiLavandula latifoliafood and beveragesPlant ScienceGeneral MedicineBiologybiology.organism_classificationchemistry.chemical_compoundTissue culturechemistryBotanyShootGeneticsmedicineMannitolAgronomy and Crop ScienceAbscisic acidmedicine.drugdescription
Abstract We studied the effect of osmotic stress (induced by the addition of mannitol to the culture medium) and abscisic acid (ABA) addition on monoterpene accumulative capability in shoot proliferating cultures of Lavandula latifolia Med. The highest shoot elongation was achieved when the elongation medium contained sucrose (3%). Increasing the medium osmolarity or adding 25–50 μM ABA in shoot elongation cultures resulted in a significant increase in monoterpene accumulation in the regenerated shoots. These monoterpenes are qualitatively similar to those produced by the parent plants. Regulation of the conditions that limit the growth in shoot proliferation cultures of L. latifolia should be useful in controlling monoterpene accumulation in shoots cultured in vitro.
year | journal | country | edition | language |
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1993-01-01 | Plant Science |