6533b836fe1ef96bd12a0889

RESEARCH PRODUCT

Spermidine and morphogenesis in single cell cultures of Sideritis angustifolia lag.

subject

description

Abstract Single cells from hypocotyl-derived callus of Sideritis angustifolia were evaluated for morphogenesis when cultured in either solidifier or liquid Murashige and Skoog medium supplemented with various concentrations of spermidine, naphthaeneacetic acid (NAA), benzyladenine (BA) and dicyclohexylamine (DCHA). Spermidine (Spd) did not replace the hormonal requirements for cell division and callus formation, but affected growth induced by NAA or BA. This polyamine increased the plating efficiency of cells cultured in the presence of NAA (0.05, 0.54 and 5.40 μM) or BA (0.40, 4.40 and 8.80 μM). In contrast, Spd reduced growth induced by 10.80 μM NAA, optimal auxin concentration for callus induction. Likewise, DCHA reduced the number of cell-derived calli obtained with 5.40 μM NAA. Data from suspension cultured cells respectively substantiated either the promoter or inhibitory effects of Spd and DCHA on cell proliferation induced by 5.40 μM NAA. To promote organogenesis, cell-derived calli were transferred to various regeneration media. Caulogenesis was only observed in those calli initially grown in the presence of Spd.