Spermidine and morphogenesis in single cell cultures of Sideritis angustifolia lag.

Abstract Single cells from hypocotyl-derived callus of Sideritis angustifolia were evaluated for morphogenesis when cultured in either solidifier or liquid Murashige and Skoog medium supplemented with various concentrations of spermidine, naphthaeneacetic acid (NAA), benzyladenine (BA) and dicyclohexylamine (DCHA). Spermidine (Spd) did not replace the hormonal requirements for cell division and callus formation, but affected growth induced by NAA or BA. This polyamine increased the plating efficiency of cells cultured in the presence of NAA (0.05, 0.54 and 5.40 μM) or BA (0.40, 4.40 and 8.80 μM). In contrast, Spd reduced growth induced by 10.80 μM NAA, optimal auxin concentration for callus…