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RESEARCH PRODUCT

Cytomics of Oxidative Stress: Probes and Problems

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Oxidative stress has been implicated in cellular senescence and aging, as well as in the onset and progression of many diverse genetic and acquired diseases and conditions. However, reactive oxygen (ROS) and nitrogen (RNS) species initiating oxidative stress also serve important regulatory roles, mediated by intercellular and intracellular signaling, adaptation to endogenous and exogenous stress, and destruction of invading pathogens. Fluorescence-based analysis of oxidative stress and related processes is an important cytomic application; almost 4000 papers were published between 1989 and 2016. To ascertain the specific role of ROS and RNS in oxidative stress studies by cytomic methodologies, it is essential to detect and characterize these species accurately. Unfortunately, the detection and quantitation of individual intracellular ROS and RNS remains a challenge, but different, complementary cytometric strategies directed toward other endpoints of oxidative stress may also be considered. In this chapter we present and briefly discuss the limitations and perspectives of such approaches.