6533b826fe1ef96bd1284917

RESEARCH PRODUCT

Insecticidal activity of Vip3Aa, Vip3Ad, Vip3Ae, and Vip3Af from Bacillus thuringiensis against lepidopteran corn pests.

Carmen Sara Hernández-rodríguezJeroen Van RieBaltasar EscrichePatricia Hernández-martínezJuan Ferré

subject

biologybusiness.industryvirusesfungiPest controlBacillus thuringiensisAgrotis ipsilonSpodopteraMothsbiology.organism_classificationMicrobiologyAffinity chromatographyBacterial ProteinsBacillus thuringiensisparasitic diseasesToxicityFall armywormAnimalsElectrophoresis Polyacrylamide GelbusinessPest Control BiologicalPolyacrylamide gel electrophoresisEcology Evolution Behavior and Systematics

description

Vip3Aa, Vip3Ad, Vip3Ae, and Vip3Af proteins from Bacillus thuringiensis were tested for their toxicity against Spodoptera frugiperda and Agrotis ipsilon. Vip3Ad was non-toxic to the two species. Vip3Ae and Vip3Af were significantly more toxic than Vip3Aa against S. frugiperda, both as protoxins and as toxins. Against A. ipsilon, Vip3Ae protoxin was more toxic than Vip3Aa and Vip3Af protoxins. Purification by metal-chelate affinity chromatography significantly affected Vip3Ae toxicity against the two insect species.

yearjournalcountryeditionlanguage
2012-11-14Journal of invertebrate pathology
10.1016/j.jip.2013.02.001https://pubmed.ncbi.nlm.nih.gov/23415860