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RESEARCH PRODUCT

Chemical Profiling of Polyfloral Belgian Honey: Ellagic Acid and Pinocembrin as Antioxidants and Chemical Markers

Izabela Jasicka-misiakPaweł KafarskiAnna PoliwodaSteven Gruyaert

subject

Antioxidantanimal structuresArticle SubjectDPPHmedicine.medical_treatmentFlavonoid01 natural sciencesHigh-performance liquid chromatographylcsh:Chemistrychemistry.chemical_compound0404 agricultural biotechnologyChemical markermedicineFood scienceChemical compositionchemistry.chemical_classificationPinocembrinChemistry010401 analytical chemistrydigestive oral and skin physiologyfungifood and beverages04 agricultural and veterinary sciencesGeneral Chemistry040401 food science0104 chemical scienceslcsh:QD1-999behavior and behavior mechanismsEllagic acid

description

Chemical profiling of northern Belgian polyfloral honeys was performed to analyse their phenolic compound content (flavonoids and phenolic acids). First, samples were subjected to a standard analysis of their physicochemical properties, and then, the phenolic fraction was isolated and analysed using a HPLC/PAD method. All of the tested honeys showed a common and specific phenolic profile that could be the basis for the differentiation of polyfloral honeys of the Antwerp region from other polyfloral honeys. Chromatographic data indicated a high content of ellagic acid (9.13–13.66 mg/100 g honey), as well as the flavonoid pinocembrin (1.60–1.85 mg/100 g honey) in these honeys. Ellagic acid, a compound with well-defined prohealth activities, might be used as a chemical marker for these honeys. With respect to total phenolic and flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were determined spectrophotometrically. The honey exhibited a moderate antioxidant activity, typical for light honeys.

10.1155/2017/5393158http://dx.doi.org/10.1155/2017/5393158