6533b827fe1ef96bd1285ce1

RESEARCH PRODUCT

Hepatocellular carcinoma after thorotrast exposure: establishment of a new cell line (Mz-Hep-1).

Karl-hermann Meyer Zum BüschenfeldeHans-peter DienesAlexander KnuthWinfried PrellwitzDieter Bitter-suermannWolfgang G. DippoldWalter Sachsse

subject

Pathologymedicine.medical_specialtyCarcinoma HepatocellularCellHuman leukocyte antigenCell Linechemistry.chemical_compoundTissue cultureCarcinoembryonic antigenLactate dehydrogenasemedicineHumansHepatitis B e AntigensHepatitis B Surface AntigensHepatologybiologyCell CycleLiver NeoplasmsAngiographyComplement System ProteinsCell cycleMiddle Agedmedicine.diseaseMolecular biologyCarcinoembryonic Antigenmedicine.anatomical_structurechemistryCell cultureHepatocellular carcinomaKaryotypingbiology.proteinMicroscopy Electron ScanningFemaleThorium Dioxidealpha-Fetoproteins

description

A human hepatoma cell line, associated with thorotrast exposure, from an hepatitis B marker-negative patient was established as a permanent cell line (Mz-Hep-1) in tissue culture. Histology of the primary tumor, as well as phase contrast, transmission and scanning electron microscopy of the cultured cells showed typical characteristics of liver cells. Mz-Hep-1 cells secreted complement components (C2, C3, C4), carcinoembryonic antigen, lactate dehydrogenase, chymotrypsin, haptoglobin and retinol-binding protein and expressed HLA-, transferrin-, blood group B-related determinants and complement component C5 and carcinoembryonic antigen on their cell surface. Mz-Hep-1 cells represent the first human hepatoma cell line, which is strongly associated with a carcinogen.

yearjournalcountryeditionlanguage
1985-11-01Hepatology (Baltimore, Md.)
10.1002/hep.1840050609https://pubmed.ncbi.nlm.nih.gov/2415435