6533b827fe1ef96bd1285d2d
RESEARCH PRODUCT
Rat CNS neurons are not yet programmed to shorten their chromatin repeat length at the end of fetal neurogenesis.
Giuseppe SalemiG. SavettieriI. Di LiegroA. CestelliDonatella FerraroDaniele Castigliasubject
Settore MED/07 - Microbiologia E Microbiologia ClinicaAgingCellular differentiationCentral nervous systemGestational AgeFetusPregnancymedicineAnimalsMitosisCells CulturedCell NucleusNeuronsFetusbiologyNeurogenesisBrainCell DifferentiationdifferentiationCell BiologyDNAneuronChromatinChromatinCell biologyRatsMolecular Weightmedicine.anatomical_structureSettore BIO/12 - Biochimica Clinica E Biologia Molecolare ClinicaImmunologybiology.proteinSettore MED/26 - NeurologiaFemaleNeuronMicrococcal nucleasedescription
Neurons from rat fetal cerebral hemispheres were grown in a synthetic medium (Maat medium), as previously described, for different periods of time. The repeat length of their chromatin was determined by micrococcal nuclease digestion and compared with that of neurons isolated from postnatal rat brain of corresponding ages. In contrast to the in vivo situation, we found that neurons, dissociated at the 16th gestational day and cultured in vitro, did not undergo the shortening of their chromatin repeat, thus indicating that, at the end of their mitotic cycles, they are not yet programmed to this event. © 1986.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1986-05-01 | Cell biology international reports |