6533b827fe1ef96bd1286474

RESEARCH PRODUCT

Effect of hydrogen peroxide on the dehydrogenase and quinone-reductase activity of irradiated Lactobacillus plantarum cells

Turs SelgaOlga MuterToms KusinsMara GrubeGunta KizaneInga BalodeAndrejs GrinbergsVasilijs BankovskisLiva MazkalninaKristine KalnenieceKarlis Svirksts

subject

0106 biological sciencesAntioxidantfood.ingredientmedicine.medical_treatmentDehydrogenase01 natural sciencesTryptic soy brothchemistry.chemical_compound0404 agricultural biotechnologyfoodQuinone Reductases010608 biotechnologymedicineAgarFood scienceHydrogen peroxidechemistry.chemical_classificationbiologyfood and beverages04 agricultural and veterinary sciencesbiology.organism_classification040401 food scienceEnzymechemistryLactobacillus plantarumFood Science

description

Abstract The resistance of lactobacilli to oxidative stress is of great importance for their applicability as probiotics. This study aimed to evaluate the response of Lactobacillus plantarum strain ATCC® 14917™, grown in either de Man, Rogosa, and Sharpe agar (MRS medium) or tryptic soy broth (TSB medium), to 1–2 mM H2O2 after the exposure to different doses of ionising radiation. Two bacterial extracellular enzyme groups, dehydrogenases (DHAs) and quinone reductases (QRs), served as the criteria of viability and antioxidant activity, respectively. The irradiated L. plantarum culture grown in TSB showed increased QR activity at irradiation doses of 2–50 Gy, with the maximum activity at 10 Gy, compared to non-irradiated cells. The QR activity of the MRS-grown culture was increased significantly (p

yearjournalcountryeditionlanguage
2020-12-01LWT
https://doi.org/10.1016/j.lwt.2020.110236