6533b827fe1ef96bd12865a3

RESEARCH PRODUCT

Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum

Kaitlyn KoenigMarcel BestRichard A. FerrieriMichael J. SchuellerAlistair RogersAlistair RogersKelly Mcdonald

subject

PhenylalanineNicotiana tabacumTrehalase activityPhenylalaninePhenylalanine ammonia-lyaseLigninBiochemistryAnalytical Chemistrychemistry.chemical_compoundPolysaccharidesTobaccoLigninOrganic chemistryHemicelluloseBiomassCarbon RadioisotopesTrehalaseFood scienceCelluloseCellulosePhenylalanine Ammonia-LyasebiologyOrganic ChemistryTrehalosefood and beveragesGeneral MedicineCarbon DioxideValidamycinbiology.organism_classificationCarbonEnzyme ActivationchemistryInositol

description

Abstract Validamycin A was used to inhibit in vivo trehalase activity in tobacco enabling the study of subsequent changes in new C partitioning into cellulosic biomass and lignin precursors. After 12-h exposure to treatment, plants were pulse labeled using radioactive 11 CO 2 , and the partitioning of isotope was traced into [ 11 C]cellulose and [ 11 C]hemicellulose, as well as into [ 11 C]phenylalanine, the precursor for lignin. Over this time course of treatment, new carbon partitioning into hemicellulose and cellulose was increased, while new carbon partitioning into phenylalanine was decreased. This trend was accompanied by a decrease in phenylalanine ammonia-lyase activity. After 4 d of exposure to validamycin A, we also measured leaf protein content and key C and N metabolite pools. Extended treatment increased foliar cellulose and starch content, decreased sucrose, and total amino acid and nitrate content, and had no effect on total protein.

yearjournalcountryeditionlanguage
2010-10-28Carbohydrate Research
https://doi.org/10.1016/j.carres.2011.01.018