Extracellular vesicles do not mediate the anti-inflammatory actions of mouse-derived adipose tissue mesenchymal stem cells secretome

6533b827fe1ef96bd1287011

RESEARCH PRODUCT

Extracellular vesicles do not mediate the anti-inflammatory actions of mouse-derived adipose tissue mesenchymal stem cells secretome

Maria Isabel Guillén María Carmen Carceller M L Gil María José Alcaraz

subject

Lipopolysaccharides Male Chemokine Lipopolysaccharide Células madre - Uso terapéutico.Adipose tissue Inflammation macrophage Article Catalysis Nitric oxide Stem cells - Therapeutic use.lcsh:Chemistry Inorganic Chemistry Mice chemistry.chemical_compound medicine Animals Macrophage Inflamación - Tratamiento.mesenchymal stem cells secretome Physical and Theoretical Chemistry Macrophages.Receptor lcsh:QH301-705.5 Molecular Biology Cells Cultured Spectroscopy biology Chemistry Inflammation - Treatment.Macrophages Organic Chemistry Mesenchymal stem cell mouse-derived adipose tissue Mesenchymal Stem Cells General Medicine Adipose tissues - Therapeutic use.Computer Science Applications Cell biology lcsh:Biology (General)lcsh:QD1-999 inflammation Macrófagos.biology.protein Tejido adiposo - Uso terapéutico.medicine.symptom extracellular vesicles

description

Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1422-0067/22/3/1375 Este artículo pertenece al número especial "The Role of Mesenchymal Stem Cells on Inflammatory and Fibrotic Diseases". Adipose tissue represents an abundant source of mesenchymal stem cells (MSC) for therapeutic purposes. Previous studies have demonstrated the anti-inflammatory potential of adipose tissue-derived MSC (ASC). Extracellular vesicles (EV) present in the conditioned medium (CM) have been shown to mediate the cytoprotective effects of human ASC secretome. Nevertheless, the role of EV in the anti-inflammatory effects of mouse-derived ASC is not known. The current study has investigated the influence of mouse-derived ASC CM and its fractions on the response of mouse-derived peritoneal macrophages against lipopolysaccharide (LPS). CM and its soluble fraction reduced the release of pro-inflammatory cytokines, adenosine triphosphate and nitric oxide in stimulated cells. They also enhanced the migration of neutrophils or monocytes, in the absence or presence of LPS, respectively, which is likely related to the presence of chemokines, and reduced the phagocytic response. The anti-inflammatory effect of CM may be dependent on the regulation of toll-like receptor 4 expression and nuclear factor- B activation. Our results demonstrate the antiinflammatory effects of mouse-derived ASC secretome in mouse-derived peritoneal macrophages stimulated with LPS and show that they are not mediated by EV.

year	journal	country	edition	language
2021-01-29

10.3390/ijms22031375 http://hdl.handle.net/10637/13660