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RESEARCH PRODUCT
Use of Early Life-Stages of Zebrafish to Assess Toxicity of Sediments Contaminated by Organotin Compounds
Tarini P. SahooAimo Oikarisubject
0301 basic medicineneural aromatase (cyp19a1b)Health Toxicology and Mutagenesista1172Soil Science010501 environmental sciences01 natural sciencessediment assay in vivotributyltin03 medical and health scienceschemistry.chemical_compoundVitellogeninIn vivoEnvironmental ChemistryBioluminescenceZebrafish0105 earth and related environmental sciencesbiologySedimentAquatic animalbiology.organism_classificationzebrafishPollutionMolecular biologyvitellogenin 1 (vtg1)030104 developmental biologychemistryEnvironmental chemistryToxicityTributyltinbiology.proteindescription
ABSTRACTThis study examined the response of early life-stages (ELS) of zebrafish to organotin-contaminated sediment from Lake Huruslahti (HL) in Central Finland. A dilution series (0, 10, 33, and 100%) of the native (HL) and the sediment spiked with tributyltin (TBT) determined a dose-response of zebrafish ELS to organotin-contaminated sediment. Sediment elutriates were assessed by bacterial bioluminescence assay and microscopical pathologies of 1–3 days post-fertilization zebrafish (1–3dpfZF). Brain aromatase (cyp19a1b) and tissue vitellogenin (vtg1) were assayed from early-juvenile zebrafish (20dpfZF) exposed to intact sediment. In vivo modulation of cyp19a1b and vtg1 transcripts in 20dpfZF was targeted to screen potential sediment contamination by TBT, an anti-estrogenic chemical. Bioluminescence assay (EC5030min = 34.6% of HL-100) determined that the HL-100 elutriate was toxic. Exposed developing embryos (1–3dpf) showed toxicities, including lowered heart rate. Results showed down-regulation of cyp19a...
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-01-05 | Soil and Sediment Contamination |