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RESEARCH PRODUCT
Phylogeny and prevalence of kobuviruses in dogs and cats in the UK
P.a. BrownAlan D RadfordL. SadlerN. Carmona-vicenteJ.y. MergaSusan DawsonAlistair C. DarbyJenny StaviskyRosalind M. GaskellJavier Buesasubject
KobuvirusPicornavirusGenotypeSequence analysisPopulationMolecular Sequence DataAichivirusCat DiseasesMicrobiologyVirusArticleDogsSequence Homology Nucleic AcidGenotypePrevalenceAnimalsDog DiseaseseducationPhylogenyeducation.field_of_studyPicornaviridae InfectionsGeneral VeterinarybiologyPicornavirusHigh-Throughput Nucleotide Sequencingvirus diseasesGeneral Medicinebiology.organism_classificationVirologyveterinary(all)United KingdomKobuvirusbiology.proteinCatsAntibodyAichi virusdescription
AbstractThe kobuviruses represent an emerging genus in the Picornaviridae. Here we have used next generation sequencing and conventional approaches to identify the first canine kobuvirus (CaKoV) from outside the USA. Phylogenetic analysis suggests that a single lineage genotype of CaKoV now exists in Europe and the USA with 94% nucleotide similarity in the coding region. CaKoV was only identified in a single case from a case–control study of canine diarrhoea, suggesting this virus was not a frequent cause of disease in this population. Attempts to grow CaKoV in cell culture failed. Sequence analysis suggested CaKoV was distinct from human Aichi virus (AiV), and unlikely to pose a significant zoonotic risk. Serosurveys by ELISA, immunofluorescence and neutralisation tests, using AiV as antigen, suggested kobuvirus infection is prevalent in dogs. In addition, IgG antibody to AiV was also detected in cat sera, indicating for the first time that cats may also be susceptible to kobuvirus infection.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-06-01 | Veterinary Microbiology |