6533b828fe1ef96bd1287923

RESEARCH PRODUCT

Characterization of epitopes recognized by Candida factor 1 and 9 antisera by use of Saccharomyces cerevisiae mnn mutants

H AtaogluRafael SentandreuJ Zueco

subject

AgglutinationAntigenicityAntigens FungalMolecular Sequence DataImmunologyMutantSaccharomyces cerevisiaeMannoseEnzyme-Linked Immunosorbent AssaySaccharomyces cerevisiaeBiologyMicrobiologyEpitopeMicrobiologyMannansEpitopeschemistry.chemical_compoundAntigenAnimalsAntibodies FungalCandidaMannanAntiserumImmune Serabiology.organism_classificationInfectious DiseasesCarbohydrate SequenceBiochemistrychemistryMutationParasitologyRabbitsResearch Article

description

The use of Saccharomyces cerevisiae mnn mutants has facilitated the study of the epitopes recognized by antisera against several antigenic factors of the genus Candida (Candida Check; Iatron Laboratories, Tokyo, Japan). We have taken advantage of the very well characterized structure of the mannans of the different mnn mutants to compare their reactivities with the factor antisera used in the identification of different species of the genus Candida. The results of this study provide evidence that one of the antigenic determinants recognized by factor 1 antisera is the O-linked mannose chains of the cell wall mannoproteins, while that recognized by factor 9 antiserum is the alpha 1-6-linked mannose backbone of the outer chain of the N-linked oligosaccharide.

yearjournalcountryeditionlanguage
1993-08-01Infection and Immunity
https://doi.org/10.1128/iai.61.8.3313-3317.1993