6533b828fe1ef96bd128793e
RESEARCH PRODUCT
Invasion of thehobo transposable element studied byin situ hybridization on polytene chromosomes ofDrosophila melanogaster
L. PascualGeorges PeriquetFrançoise LemeunierMáximo Ibo GalindoV. Ladevèzesubject
Transposable elementEmbryo NonmammalianCentromerePlant ScienceIn situ hybridizationGenomeChromosomesPlasmidGeneticsMelanogasterAnimalsIn Situ HybridizationGeneticsGenomePolytene chromosomebiologyChromosome MappingChromosomeGeneral Medicinebiology.organism_classificationBlotting SouthernDrosophila melanogasterInsect ScienceDNA Transposable ElementsAnimal Science and ZoologyDrosophila melanogasterdescription
The invasion kinetics of hobo transposable element in the Drosophila melanogaster genome was studied by in situ hybridization on the polytene chromosomes. Six independent lines of Drosophila melanogaster flies that had been previously transformed by microinjection of the pHFL1 plasmid containing a complete hobo element were followed over 50 generations. We observed that hobo elements were scattered on each of the chromosome arms, with more insertion sites on the 3R arm. The total number of insertion sites remains quite small, between four and six, at generation 52. On the 2R arm, a short inversion appeared once at generation 52. Most of the integration sites reported here were already described for several transposons but some of them appear to be hotspots for hobo elements.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-02-01 | Genetica |