6533b828fe1ef96bd1287a83

RESEARCH PRODUCT

Expression of the pea S -adenosylmethionine decarboxylase gene is involved in developmental and environmental responses

Pedro CarrascoFrancisco Marco

subject

Adenosylmethionine DecarboxylaseDNA ComplementaryCarboxy-lyasesMolecular Sequence DataSpermineSaccharomyces cerevisiaePlant ScienceEnvironmentBiologyGene Expression Regulation EnzymologicPisumchemistry.chemical_compoundOzoneGene Expression Regulation PlantGene expressionGeneticsAmino Acid SequenceGeneSequence Homology Amino AcidReproductionGenetic Complementation TestPeasGene Expression Regulation Developmentalfood and beveragesSequence Analysis DNAbiology.organism_classificationCell biologyPlant LeavesBiochemistrychemistryAdenosylmethionine decarboxylaseFruitCallusMutationSperminePolyamineSequence AlignmentCell DivisionPlant Shoots

description

A cDNA, able to complement the S-adenosyl-L-methionine decarboxylase (SAMdC; EC 4.1.1.50)-defective yeast strain Y342, has been isolated from pea (Pisum sativum L.). Expression of the SAMdC gene was characterised during pea development. Northern analysis showed a differential expression of the pea SAMdC gene in vegetative and reproductive tissues. The highest SAMdC mRNA levels were found in undifferentiated callus and tissues with high rates of cell division, and at the onset of fruit development. SAMdC expression was also induced in senescing ovaries, probably in relation to an accumulation of spermine during ovary senescence. Finally, the levels of SAMdC transcripts in leaves and shoots were differentially affected by ozone treatment.

https://doi.org/10.1007/s004250100653