6533b828fe1ef96bd1288c85
RESEARCH PRODUCT
Derivatization techniques for automated chromatographic analysis of amphetamine usingo-phthaldialdehyde: A comparative study
Pilar Campíns-falcóRosa Herráez-hernándezsubject
Detection limitReproducibilityAnalyteChromatographyOrganic ChemistryClinical BiochemistryBiochemistryHigh-performance liquid chromatographyAnalytical Chemistrychemistry.chemical_compoundchemistrymedicineSample preparationAmphetamineDerivatizationQuantitative analysis (chemistry)medicine.drugdescription
The potential of different chromatographic systems for automated, on-line analysis of amphetamine in biological fluids is illustrated. The various systems integrate analyte purification and enrichment, separation, derivatization in different derivatization modes witho-phthaldialdehyde (OPA) and N-acetyl-L-cysteine (NAC), and fluorimetric detection. The reliability of the systems has been tested by analysing urine and plasma samples containing amphetamine in the 0.1–20.0 μg mL−1 range. Pre, on and post-column derivatization strategies are compared in terms of their instrumental requirements, selectivity, sensitivity, linearity and reproducibility.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-08-01 | Chromatographia |