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RESEARCH PRODUCT
Quantitative analysis of broad molecular weight distributions obtained by matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry
Jochen SpickermannKai MartinAxel H. E. MüllerHans Joachim RäderKlaus MüllenHelmut SchlaadRalph-peter Krügersubject
chemistry.chemical_classificationMALDI imagingChemistryDesorptionAnalytical chemistryPolymerTime-of-flight mass spectrometryMass spectrometryQuantitative analysis (chemistry)Capillary electrophoresis–mass spectrometrySpectroscopySurface-enhanced laser desorption/ionizationdescription
In order to quantify the error of matrix-assisted laser desorption ionisation (MALDI) time-of-flight (TOF) mass spectrometry in the determination of broad molecular weight distributions, different mixtures by weight of two poly(methyl methacrylate) standards were prepared. These mixtures, with well-defined bimodal molecular weight distributions were analysed by MALDI-TOF mass spectrometry using different matrices (2,4,6-trihydroxyacetophenone and 2,5-dihydroxybenzoic acid) and different cations (Li+, Na+, K+, Rb+ and Cs+) for doping the analyte. From the MALDI-TOF mass spectrometric data, the weight fractions of the two polymers of all mixtures were determined and compared to the values measured by size-exclusion chromatography. The results show that it is possible to obtain the correct Mn and Mw values with 2,5-dihydroxybenzoic acid as matrix and K+ as cationisation metal.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-01-01 | European Journal of Mass Spectrometry |