6533b828fe1ef96bd1288f9a
RESEARCH PRODUCT
Distribution of VP7 serotypes and VP4 genotypes among rotavirus strains recovered from Italian children with diarrhea
Antonio CascioEsmeralda VizziR. Di StefanoSerenella AristaDonatella Ferrarosubject
DiarrheaSerotypemedicine.medical_specialtyGenotypeReoviridaeBiologymedicine.disease_causePolymerase Chain ReactionRotavirus Infectionslaw.inventionCapsidMedical microbiologylawVirologyRotavirusGenotypemedicineHumansUNIQUE VP4SerotypingChildAntigens ViralPolymerase chain reactionMolecular epidemiologyGeneral MedicinePOLYMERASE CHAIN-REACTIONbiology.organism_classificationVirologyGastroenteritisDiarrhearotavirusItalyChild PreschoolRNA ViralCapsid Proteinsmedicine.symptomdescription
108 rotavirus strains obtained from children with diarrhea hospitalized in Palermo, Italy, in the years 1990-1994, were examined by seminested PCR to study the relative frequency and distribution of the four most common alleles of the gene 4. Such strains were selected from 344 human rotavirus strains recovered in palermo during those years after characterization by electropherotyping, subgrouping and G serotyping. One hundred and seven of the 108 strains could be classified into P types, the P[8], G1 (38.3%) and the P[8], G4 (52.3%) types being predominant. The unique strain whose P genotype could not be identified showed an unusual combination of long migration electrophoretic pattern and subgroup I specificity.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-04-06 | Archives of Virology |