6533b828fe1ef96bd1288fcd
RESEARCH PRODUCT
Advanced fluorescence microscopy for in vivo imaging of neuronal activity
Anna Letizia Allegra MascaroFrancesco S. PavoneLeonardo SacconiLudovico SilvestriGiuseppe Sancataldosubject
Flexibility (engineering)0303 health sciencesBrain activity and meditationComputer science01 natural sciencesAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic Materials010309 optics03 medical and health scienceslight-sheet microscopy; field-of-view; cellular-resolution; adaptive optics; multiphoton microscopy; GRID CELLS; HIGH-SPEED; LONG-TERM; 2-PHOTON; DEEPLight sheet fluorescence microscopy0103 physical sciencesFluorescence microscopePremovement neuronal activityIn vivo microscopyOptics In vivo imaging MicroscopyNeurosciencePreclinical imagingBrain function030304 developmental biologydescription
Brain function emerges from the coordinated activity, over time, of large neuronal populations placed in different brain regions. Understanding the relationships of these specific areas and disentangling the contributions of individual neurons to overall function remain central goals for neuroscience. In this scenario, fluorescence microscopy has been proved as the tool of choice for in vivo recording of brain activity. Optical advances combined with genetically encoded indicators allow a large flexibility in terms of spatiotemporal resolution and field of view while keeping invasiveness in living animals to a minimum. Here we describe the latest advancements in the field of linear and nonlinear optical microscopy with special attention to the exploration of brain functionality of model animals. The present review aims to guide the reader through the main optical systems in the field toward future directions for in vivo microscopy applications. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2019-05-31 | Optica |