6533b828fe1ef96bd1288ffb
RESEARCH PRODUCT
Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection
Juliette GuibertSylvie EpelboinAida KaffelDominique De ZieglerPatricia FauqueCatherine PatratJean-philippe WolfLucie DelarochePierre Jouannetsubject
PregnancyArticle Subjectbusiness.industryMetaphase iiurogenital systemmedicine.medical_treatmentObstetrics and GynecologyOocytemedicine.diseaselcsh:Gynecology and obstetricsIntracytoplasmic sperm injectionAndrologymedicine.anatomical_structureDenudationMedicinebusinessMicroinjectionIncubationFertilisationreproductive and urinary physiologylcsh:RG1-991Research Articledescription
Objectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes.Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age years, male factor infertility, long protocol using GnRH agonist and rFSH for ovarian stimulation, and use of freshly ejaculated sperm (). Several ICSI parameters were analyzed according to the time between oocyte retrieval and denudation () and the time between denudation and ICSI () using a statistical logistic regression analysis.Results. Neither nor had a significant influence on the Metaphase II (MII) rate but the fertilisation rate (FR) showed a significant improvement when was longer (optimal results at hours) while FR significantly decreased with the increase of . Optimal implantation (IR) and pregnancy (PR) rates were obtained when was around 2 hours.Conclusion. Incubation of oocytes around 2 hours between retrieval and denudation may not increase MII rate but appears to lead to the optimal combination of FR and IR.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-07-20 | Obstetrics and Gynecology International |