6533b829fe1ef96bd1289851

RESEARCH PRODUCT

Photoactivatable Adhesive Ligands for Light-Guided Neuronal Growth

Aránzazu Del CampoAránzazu Del CampoShifang ZhaoShifang ZhaoWenqiang FanAleeza FarrukhAleeza FarrukhMarcelo SaliernoMarcelo SaliernoJulieta I. Paez

subject

0301 basic medicineGUIDED AXON GROWTHNeuritePeptidomimeticNeurogenesisPeptideBiocompatible Materials02 engineering and technologyNEUROCHEMISTRYLigandsBiochemistryPHOTOTRIGGERED CELL ADHESIONCell membrane03 medical and health sciencesLamininmedicineCell AdhesionAnimalsAmino Acid SequenceCell adhesionMolecular BiologyCells Culturedchemistry.chemical_classificationNeuronsPhotolysisbiologyChemistryCELL ADHESIONOrganic ChemistryCiencias QuímicasAdhesion021001 nanoscience & nanotechnologyIn vitroLAMININ PEPTIDOMIMETICSMice Inbred C57BL030104 developmental biologymedicine.anatomical_structureQuímica OrgánicaBiophysicsbiology.proteinMolecular MedicineLaminin0210 nano-technologyPeptidesCIENCIAS NATURALES Y EXACTAS

description

Neuro-regeneration after trauma requires growth and reconnection of neurons to reestablish information flow in particular directions across the damaged tissue. To support this process, biomaterials for nerve tissue regeneration need to provide spatial information to adhesion receptors on the cell membrane and to provide directionality to growing neurites. Here, photoactivatable adhesive peptides based on the CASIKVAVSADR laminin peptidomimetic are presented and applied to spatiotemporal control of neuronal growth to biomaterials in vitro. The introduction of a photoremovable group [6-nitroveratryl (NVOC), 3-(4,5-dimethoxy-2-nitrophenyl)butan-2-yl (DMNPB), or 2,2′-((3′-(1-hydroxypropan-2-yl)-4′-nitro-[1,1′-biphenyl]-4-yl)azanediyl)bis(ethan-1-ol) (HANBP)] at the amino terminal group of the K residue temporally inhibited the activity of the peptide. The bioactivity was regained through controlled light exposure. When used in neuronal culture substrates, the peptides allowed light-based control of the attachment and differentiation of neuronal cells. Site-selective irradiation activated adhesion and differentiation cues and guided seeded neurons to grow in predefined patterns. This is the first demonstration of ligand-based light-controlled interaction between neuronal cells and biomaterials. Fil: Farrukh, Aleeza. Leibniz Institute for New Materials; Alemania. Max Planck Graduate Center; Alemania Fil: Fan, Wenqiang. Johannes Gutenberg Universitat Mainz; Alemania Fil: Zhao, Shifang. Universitat Saarland; Alemania. Leibniz Institute for New Materials; Alemania Fil: Salierno, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina. Johannes Gutenberg Universitat Mainz; Alemania Fil: Paez, Julieta Irene. Leibniz Institute for New Materials; Alemania Fil: del Campo, Aránzazu. Universitat Saarland; Alemania. Leibniz Institute for New Materials; Alemania

yearjournalcountryeditionlanguage
2018-01-01
https://dx.doi.org/10.22028/d291-29490