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RESEARCH PRODUCT
Study of oligogalacturonides-triggered Nitric Oxide (NO) production provokes new questioning about the origin of NO biosynthesis in plants
David WendehenneSumaira RasulSylvain Jeandrozsubject
Arabidopsis thalianaMutantArabidopsisOligosaccharidesPlant ScienceNitrate reductaseModels BiologicalNitric oxidechemistry.chemical_compoundBiosynthesisL-NAMEGene Expression Regulation PlantPlant defenseArabidopsisPlant defense against herbivoryArabidopsis thaliana[SDV.BV]Life Sciences [q-bio]/Vegetal Biologybiologyfungifood and beveragesNitric oxideBiotic stressbiology.organism_classificationOligogalacturonidesArticle AddendumNG-Nitroarginine Methyl EsterBiochemistrychemistryNitrate reductasedescription
Addendum to: Rasul S, Dubreuil-Maurizi C, Lamotte O, Koen E, Poinssot B, Alcaraz G, et al. Nitric oxide production mediates oligogalacturonide-triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana. Plant Cell Environ 2012; PMID:22394204; http://dx.doi. org/10.1111/j.1365-3040.2012.02505.x.; International audience; We investigated the production and function of nitric oxide (NO) in Arabidopsis thaliana leaf discs as well as whole plants elicited by oligogalacturonides (OGs). Using genetic, biochemical and pharmacological approaches, we provided evidence that OGs induced a Nitrate Reductase (NR)-dependent NO production together with an increased NR activity and NR transcripts accumulation. In addition, NO production was sensitive to the mammalian NOS inhibitor L-NAME. Intriguingly, L-NAME impaired OG-induced NR activity and did not further affect the remaining OG-induced NO production in the nia1nia2 mutant. These data suggest that the L-arginine and NR pathways, co-involved in NO production, do not work independently. Taking account these new data, we propose scenarios to explain NO production in response to biotic stress.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-10-28 |