6533b829fe1ef96bd1289adc
RESEARCH PRODUCT
Individual stereoisomers of phosphinic dipeptide inhibitor of leucine aminopeptidase
Michael LämmerhoferMałgorzata PawełczakPaweł KafarskiPaweł KafarskiWolfgang LindnerArthur Muchasubject
StereochemistryCarboxylic acidleucine aminopeptidaseClinical BiochemistryPharmaceutical ScienceBiochemistryChemical synthesisLeucyl Aminopeptidasechemistry.chemical_compoundinhibitorsDrug DiscoveryMolecular Biologychemistry.chemical_classificationDipeptideMolecular StructureChemistryOrganic Chemistryphosphinic dipeptidesDiastereomerAbsolute configurationStereoisomerismDipeptidesPhosphinic Acidsstereoselective separationMolecular MedicineStereoselectivityLeucineEnantiomerdescription
Abstract Individual stereoisomers of the phosphinic pseudodipeptide hPheψ[P(O)(OH)CH2]Phe were obtained by stereoselective liquid chromatographic separation as N- and C-terminally protected derivative on quinidine carbamate modified silica stationary phase. The stereoisomeric purity, exceeding 95% for each fraction, was determined before and after deprotection using two independent methods. The absolute configuration was rationally assigned by application of enantiomerically pure phosphinic acid substrates in the synthetic procedure and correlation with biological activity of the products. Substantial differences in inhibition of leucine aminopeptidase by the individual isomers revealed novel insights into potency of the recently developed and remarkably effective compound. Graphical abstract First successful separation of all four stereoisomers of a phosphinic acid pseudodipeptide allowed to determine the inhibitory activity of the homophenylalanyl-phenylalanine analogue towards leucine aminopeptidase stereoselectively.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-01-10 | Bioorganic & Medicinal Chemistry Letters |