6533b829fe1ef96bd1289b1f
RESEARCH PRODUCT
Establishment and characterization of a nontumorigenic cell line derived from a human hepatocellular adenoma expressing hepatocyte-specific markers.
Fataneh NiketeghadFranz OeschPablo SteinbergPeter SchirmacherRosario HeckHans Peter DienesSvetlana RadaevaPeter BannaschClaudia Schlegersubject
Mice NudeBiologymedicine.disease_causeAdenoma Liver CellCytokeratinMicemedicineBiomarkers TumorTumor Cells CulturedAnimalsHumansCellular SenescenceEndoplasmic reticulumLiver NeoplasmsContact inhibitionEpithelial CellsCell BiologySequence Analysis DNAHepatocellular adenomamedicine.diseaseGenes p53Cell biologymedicine.anatomical_structureCytoplasmCell cultureOrgan SpecificityHepatocyteKaryotypingCarcinogenesisdescription
In the present study the establishment and characterization of a nontumorigenic liver epithelial cell line (HACL-1) derived from a human hepatocellular adenoma is described. The HACL-1 cells have a finite life span (i.e., they proliferate for a period of 2 months and then senesce), show cell-cell contact inhibition, do not grow in soft agar, are not tumorigenic when injected in nude mice, and possess a normal diploid karyotype. The cultured cells resemble hepatocytes, but exhibit some features of dedifferentiation. At the ultrastructural level the cells are endowed with round or oval nuclei, abundant cytoplasmic organelles, and varying amounts of glycogen. The rough endoplasmic reticulum is disorganized, while peroxisomes and matrix granules within mitochondria are lacking. HACL-1 cells are cytokeratin 18-positive as well as (transiently) albumin- and alpha-fetoprotein-positive, but do not express cytokeratin 19. Furthermore, no mutations were observed in exons 5-8 of the tumor suppressor gene p53. Taken together these results show that HACL-1 cells are nontumorigenic proliferating liver epithelial cells, which might prove to be of great value in future studies on diverse aspects of human liver cell biology and carcinogenesis.
year | journal | country | edition | language |
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1997-11-21 | Experimental cell research |