6533b829fe1ef96bd128a56a

RESEARCH PRODUCT

Partially Reversible Adsorption of Annexin A1 on POPC/POPS Bilayers Investigated by QCM Measurements, SFM, and DMC Simulations

Katja KastlVolker GerkeClaudia SteinemEike LüthgensManuela MenkeAndreas JanshoffSimon Faiß

subject

Time FactorsSurface PropertiesLipid BilayersKineticsAnalytical chemistryBiosensing TechniquesPhosphatidylserinesMicroscopy Atomic ForceBiochemistrychemistry.chemical_compoundAdsorptionPhase (matter)MicroscopyComputer SimulationParticle SizeLipid bilayerMolecular BiologyPOPCAnnexin A1ChemistryOrganic Chemistrytechnology industry and agricultureMembranes ArtificialQuartz crystal microbalanceMembraneMicroscopy FluorescencePhosphatidylcholinesMolecular Medicinelipids (amino acids peptides and proteins)AdsorptionStress MechanicalMonte Carlo Method

description

The kinetics of annexin A1 binding to solid-supported lipid bilayers consisting of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoserine (POPS; 4:1) has been investigated as a function of the calcium ion concentration in the bulk phase. Quartz crystal microbalance measurements in conjunction with scanning force microscopy, fluorescence microscopy, and computer simulations indicate that at a given Ca2+ concentration annexin A1 adsorbs irreversibly on membrane domains enriched in POPS. By contrast, annexin A1 adsorbs reversibly on the POPC-enriched phase, which is composed of single POPS molecules embedded within a POPC matrix. The overall area occupied by the POPS-enriched phase is controlled by the CaCl2 concentration. Monte Carlo simulations suggest that the area of the POPS-enriched phase increases by a factor of 7 when the Ca2+ concentration is changed from 0.01 to 1 mM.

yearjournalcountryeditionlanguage
2005-11-25ChemBioChem
https://doi.org/10.1002/cbic.200500265