6533b829fe1ef96bd128ae99
RESEARCH PRODUCT
Self-assembling and auto-crosslinkable hyaluronic acid hydrogels with a fibrillar structure
Filippo CalascibettaGiovanna PitarresiGaetano GiammonaFabio Salvatore PalumboAntonella Albanesesubject
Magnetic Resonance SpectroscopyTime FactorsMaterials scienceCell SurvivalPolymersBiomedical EngineeringCystineStackingBiochemistryFluorescence spectroscopyPhosphatesBiomaterialschemistry.chemical_compoundMaterials TestingSpectroscopy Fourier Transform InfraredPolymer chemistryHyaluronic acidHumansDisulfidesHyaluronic AcidDerivatizationMolecular BiologyCell Proliferationchemistry.chemical_classificationHydrogelsGeneral MedicinePolymerFibroblastsHydrogen-Ion ConcentrationOxygenCross-Linking ReagentschemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoSelf-healing hydrogelsMicroscopy Electron ScanningThiolCystineself assembling tissue engineering hyaluronic acid cell entrapmentBiotechnologydescription
Abstract A hyaluronic acid derivative bearing pendant l -benzoyl-cysteine portions (with a derivatization degree equal to 10 mol.%) was synthesized by linking N,N′-dibenzoyl- l -cystine to the polysaccharide and then reducing its disulfide bridge to thiol groups. The formation of π–π stacking interactions between the benzoyl moieties was studied by fluorescence spectroscopy as a function of polymer concentration and oxidation time. The efficiency of oxidation of thiol groups to disulfide bridges occurring in phosphate buffer pH 7.4, was determined by colorimetric assays. The hydrogel formed by means of oxidative crosslinking has shown the presence of fibrillar aggregates as detected by light and scanning electron microscopy. Human derm fibroblasts were encapsulated into hydrogel-forming solution, and their ability to proliferate was tested during 3 days of culture.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-11-04 |