6533b829fe1ef96bd128afb3
RESEARCH PRODUCT
pilF polymorphism-based PCR to distinguish Vibrio vulnificus strains potentially dangerous to public health
Eva SanjuánCarmen AmaroFrancisco J. RoigA. Llorenssubject
DNA BacterialMalemedicine.medical_specialtyVirulence FactorsMolecular Sequence DataVirulenceMicrobiologiaPublic Health MicrobiologyVibrio vulnificusBiologyPolymerase Chain ReactionApplied Microbiology and Biotechnologylaw.inventionMicrobiologyMiceBacterial ProteinslawVibrionaceaeVibrio InfectionsmedicineAnimalsHumansVibrio vulnificusGenePolymerase chain reactionGeneticsBacteriological TechniquesMice Inbred BALB CPolymorphism GeneticBase SequenceVirulenceEcologyPublic healthFishesSequence Analysis DNAbiology.organism_classificationPathogenicityVibrio InfectionsFimbriae ProteinsSequence AlignmentFood ScienceBiotechnologydescription
ABSTRACT Vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. In this report, we describe a PCR-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. Discrimination is based on the amplification of a variable region located within the gene pilF , which seems to be associated with potential human pathogenicity, regardless of the biotype of the strain.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2010-01-01 |