6533b82afe1ef96bd128b799

RESEARCH PRODUCT

Polysialic acid can mediate membrane interactions by interacting with phospholipids

Teresa JanasKrzysztof NowotarskiTadeusz Janas

subject

LiposomeChemistryPolysialic acidVesicleOrganic ChemistryPhospholipidMembranes ArtificialCell BiologyHydrogen-Ion ConcentrationBiochemistrychemistry.chemical_compoundSpectrometry FluorescenceMembraneMicroscopy Electron TransmissionBiochemistryPhosphatidylcholineSialic AcidsBiophysicsNeural cell adhesion moleculeAdsorptionLipid bilayerMolecular BiologyPhospholipids

description

Polysialic acid (polySia) is expressed on the surface of neural cells, neuroinvasive bacterial cells and several tumor cells. PolySia chains attached to NCAM can influence both trans interactions between membranes of two cells and cis interactions. Here, we report on the involvement of phospholipids in regulation of membrane interactions by polySia. The pH at the surface of liposomes, specific molecular area of phosphatidylcholine molecules, phase transition of DPPC bilayers, cyclic voltammograms of BLMs, and electron micrographs of phosphatidylcholine vesicles were studied after addition of polysialic acid free in solution. The results indicate that polySia chains can associate with phosphatidylcholine bilayers, incorporate into the polar part of a phospholipid monolayer, modulate cis interactions between phosphatidylcholine molecules, and facilitate trans interactions between apposing phospholipid vesicles. These observations imply that polySia attached to NCAM or to lipids can behave similarly.

yearjournalcountryeditionlanguage
2009-07-13Chemistry and Physics of Lipids
https://doi.org/10.1016/j.chemphyslip.2009.12.003